Quantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum sheds light on the pathogenesis of canine angiostrongylosis

Abstract Blood contains hundreds of proteins, reflecting ongoing cellular processes and immune reactions. Infections with the blood-dwelling cardiopulmonary nematode Angiostrongylus vasorum in dogs manifest a broad spectrum clinical signs including respiratory distress, bleeding diathesis neurological signs, are associated perturbed blood protein profile dogs. However, current knowledge does not completely explain observed pathologies induced by A. infections, disorders. Using sera from experimentally infected dogs, dog serum proteome was analysed quantitative mass spectrometry methods over several time points before after inoculation. Following computational analysis, we identified 139 up- downregulated proteins infection (log2 ratio cut-off ≥ 1.0; q-value ≤ 0.05). Among upregulated were chitinase 3-like 1 pulmonary surfactant-associated B fold-changes 5). Pathway enrichment revealed complement (especially lectin pathway) coagulation cascades as significantly affected upon analysis proteins. them mannan-binding serine peptidases, ficolin, factor XIII-B. These results bring new elements towards understanding underlying pathomechanisms diatheses some -infected